Effects of fluidization on empty microcapsule integrity within the bioreactors.
(A) Microcapsule retention rates of the DMFBB and FBB operated at 90 and 150 ml/min. (B) Swelling rates (%) of microcapsules in the DMFBB and FBB operated at 90 and 150ml/min. (C) Percentages of broken microcapsules in the DMFBB and FBB operated at 90 and 150 ml/min. The following results were obtained: (A): When the DMFBB was operated at 90 ml/min, the rate of microcapsule retention was 99.8% compared to 91.74% in the FBB at day 1 (p = 0.0025), 98.08% compared to 90.3% at day 2 (p = 0.0022), and 96.55% compared to 87.68% at day 3 (p = 0.0024).When the DMFBB was operated at 150 ml/min, the rate of microcapsule retention was 99.78% compared to 92.14% in the FBB at day 1 (p = 0.0051), 97.49% compared to 87.98% at day 2 (p = 0.0014), and 94.71% compared to 84.95% at day 3 (p = 0.0008). (B): When the DMFBB was operated at 90 ml/min, the swelling rate (%) of microcapsules was 7.7% compared to 15.81% in the FBB at day 1 (P = 0.0176), 11.59% compared to 19.64% at day 2 (p = 0.0027), and 12.8% compared to 34.81% at day 3 (p = 0.0005). When the DMFBB was operated at 150 ml/min, the swelling rate of microcapsules was 5.49% compared to 23.49% in the FBB at day 1 (p = 0.0328), 14.35% compared to 27.59% at day 2 (p = 0.0241), and 30.11% compared to 36.66% at day 3 (p = 0.3258). (C): When the DMFBB was operated at 90 ml/min, the percentage of broken microcapsules in the DMFBB was 1.6% compared to 3.2% in the FBB at day 1 (p = 0.0095), 4.8% compared to 7.4% at day 2 (p = 0.0117), and 6.4% compared to 11.6% at day 3 (p = 0.0017). When DMFBB was operated at 150 ml/min, the percentage of broken microcapsules in the DMFBB was 1.9% compared to 4.2% in the FBB at day 1 (p = 0.0042), 5.6% compared to 9.8% at day 2 (p = 0.0114), and 7.4% compared to 11.8% at day 3 (p = 0.0031).
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